PHARMACOLOGY FACULTY
Selected Publications
  • Kowalczyk, M.C., Spears E., Narog M., Zoltaszek R., Kowalczyk P., Hanausek M., Yoshimi N., Slaga T.J., Walaszek, Z. (2011) Modulation of biomarkers related to tumor initiation and promotion in mouse skin by a natural β-glucuronidase inhibitor and its precursors. Clin Oncol Rep. Sep; 26(3):551-6. PubMed PMID:21687956.
  • Hanausek M., Spears E., Walaszek Z., Kowalczyk M.C., Kowalczyk P., Wendel C., Slaga T.J., (2011) Inhibition of murine skin carcinogenesis by freeze-dried grape powder and other grape-derived major antioxidants. Nutr Cancer Jan; 63(1):28-38. PubMed PMID:21108125
  • Kinjo T., Kowalczyk P., Kowalczyk M.C., Walaszek Z., Slaga T.J., Hanausek, M. (2010) Effects of desipramine on the cell cycle and apoptosis in Ca3/7 mouse skin squamous carcinoma cells. Int J Mol Med Jun; 25(6):861-7. PubMed PMID:20428789
  • Kowalczyk M.C., Kowalczyk P., Tolstykh O., Hanausek M., Walaszek Z., Slaga T.J., (2010) Synergistic effects of combined phytochemicals and skin cancer prevention in SENCAR mice. Cancer Prev Res (Phila) Feb; 3(2):170-8. PubMed PMID:20103723
  • Kinjo T., Kowalczyk P., Kowalczyk M.C., Walaszek Z., Nishimaki T., Slaga T.J., Hanausek, M. (2009) Desipramine inhibits the growth of a mouse skin squamous cell carcinoma cell line and affects glucocorticoid receptor-mediated transcription. Mol Carcinog Dec;48(12):1123-30. PubMed PMID:19575421
  • Kowalczyk M.C., Walaszek Z., Kowalczyk P., Kinjo T., Hanausek M., Slaga T.J., (2009) Differential effects of several phytochemicals and their derivatives on murine keratinocytes in vitro and in vivo: implications for skin cancer prevention. Carcinogenesis Jun;30(6):1008-15. PubMed PMID:19329757
  • Kowalczyk P., Kinjo T., Kowalczyk M.C., Walaszek Z., Hanausek M., Slaga T.J., (2009) Effect of phosphodiesterase antagonists on glucocorticoid mediated growth inhibition in murine skin cell lines. Eur J Pharmacol May21:610(1-3):29-36. PubMed PMID:19306867
Thomas Slaga
 

Thomas Slaga

Professor of Pharmacology
Ph.D., University of Arkansas Medical Center - Little Rock

Office: 210-567-4231
Email: slagat@uthscsa.edu

 

View video introduction to Dr. Slaga's lab

 

Keywords

glucocorticoid hormones, carcinogen, carcinogenesis, tumor, papillomas, squamous cell carcinomas, skin cancer

 

Research Summary

The research in Dr. Thomas Slaga's laboratory is focused on glucocorticoid hormones (GC), very potent inhibitors of physiological DNA synthesis in keratinocytes in vivo. These hormones are also very effective in preventing carcinogen- and tumor promoter-induced skin hyperplasia, inflammation, and mouse skin tumor formation when applied to skin together with a carcinogen or a tumor promoter. We and others have shown, however, that the GC do not affect the growth of either established papillomas, squamous cell carcinomas (SCC), or transformed keratinocytes in vitro. In addition, we recently found that the GC do not affect glucocorticoid-responsive genes in transformed keratinocytes both in vitro and in vivo. We have generated skin-targeted transgenic mice over-expressing the GR under the control of the keratin 5 (K5) promoter. These adult transgenic mice have impaired proliferative and inflammatory responses to skin tumor promoters. Our initial studies showed that the K5.GR transgenic animals are resistant to ras-induced tumorigenesis. The constitutively nuclear overexpression and activation of the GR in the epidermis dramatically inhibited skin tumor development in K5.GR/ras+ double transgenic mice in terms of number of animals that develop tumors, number of tumors per animal, and tumor size. In another study we plan to determine the mechanism(s) of synergistic action of the natural source compounds, known to inhibit one or more stages of skin carcinogenesis, i.e., initiation and promotion/progression. The concurrent topical and systemic (i.e., dietary) treatment with selected natural source inhibitors of different stages of skin carcinogenesis result in synergistic effects leading to more efficient prevention of skin cancer. The natural source inhibitors to be tested include ellagic acid, imperatorin from the family of coumarins, proanthocyanidin B-2-gallate, (-)-epigallocatechin from the family of green tea polyphenols, N-acetylcysteine, calcium D-glucarate, lycopene, carnosol and ursolic acid from rosemary extract, and resveratrol. We propose to initially utilize a number of very predictive short-term in vitro and in vivo tests in order to identify the
mechanism(s) and to differentiate the potencies of selected inhibitors at various concentrations under standard conditions. The most effective compounds will then be studied in long-term tumor experiments utilizing a 7,12-dimethylbenz[a]anthracene (DMBA)-induced 12-O-tetradecanoylphorbol-13-acetate (TPA)-promoted multistage carcinogenesis model in SENCAR mice.


• Lab Personnel •

Huiyun Liang, Ph.D. - Instructor/Research (C&SB)

Anna Mancha - Graduate Student